Accompanied by party leaders Dimple Yadav, wife of SP chief Akhilesh Yadav, Rajendra Chowdhury and Kironmay Nanda, besides businessman Subrata Roy Sahara, Jaya Bachchan, who turns 70 next month, filed her nomination at the Central Hall of the state assembly.
Since the trouble in Uttar Pradesh's ruling party became public, Akhilesh has been insisting on sidelining or expulsion of Singh.
SP has fielded Shivpal from Jaswant Nagar seat in Etawah district.
The party's constitution was amended to extend the tenure of the party chief to five years from the existing three years.
Akhilesh said that the defeat of the Dalit candidate would cement his party's relations with Mayawati-led BSP.
Party patriarch Mulayam Singh Yadav and warring uncle Shivpal were conspicuous in their absence.
A day ahead of the nationwide protests planned by the opposition against demonetisation, Prime Minister Narendra Modi defended the move and warned unscrupulous people using the Jan Dhan accounts of the poor to launder their black money of strict action.