Duenas Nevado is to be fired with immediate effect from his Barloworld team after his positive test, team manager Claudio Corti said on Thursday.
"He is to be fired today," Corti told reporters.
French prosecutors said on Thursday bags of blood and syringes had been found in Duenas Nevado's suitcases.
The Tour had been scandal-free until last Friday, when it was announced that Beltran of the Liquigas team had tested positive for EPO after the first stage.
Last year's Tour was marred by doping scandals.
Then overall leader Michael Rasmussen of Denmark was kicked out of the race for lying about his training whereabouts and Kazakh Alexandre Vinokourov tested positive for blood doping.
His Astana team were asked to leave the race.
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