The Gandhinagar-Mumbai Vande Bharat Superfast Express hit a cattle between Udvada and Vapi stations in Gujarat on Thursday evening, a railway official said.
The collision caused a minor dent to the train's front panel, he added.
This is the fourth such incident involving the semi-high speed train on the route since it began operating two months ago.
Sumit Thakur, chief public relations officer of Western Railway, said the incident occurred near level crossing gate no 87 between Udvada and Vapi around 6.23 pm.
"There was a minor dent in the front portion with no operational issues. The dent will be attended to tonight," he said.
After a brief halt, the train resumed journey at 6.35 pm.