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Tournament favourite Serena Williams withdrew from the Italian Open on Saturday with a lower back injury, the WTA said.
The American had been due to play China's Li Na in the first semi-final.
French Open holder Li will now go through to play either Maria Sharapova of Russia or Germany's Angelique Kerber.
Williams, who has won clay-court titles in Charleston and Madrid in the past month, was expected to give a news conference later on Saturday to explain the extent of the problem.
