The brawl took place as Khairiah Saber, Osama Bin Laden's eldest wife branded her younger rival Amal Ahmed al-Sadah, a slut, sparking off a vicious catfight in the prison.
The brawling pair, kept in custody since the world's most wanted terrorist was killed last May, had to be pulled apart by shocked guards.
Guards were under "strict instructions" never to leave oldest wife Khairiah, 61, alone with youngest Amal, 29, in case one of them kills the other, the Sun reported.
A commander from Pakistani spy agency Inter-Services Intelligence told the daily how the amazing bust-up was sparked by Amal's suspicions that Khairiah had betrayed five-times married bin Laden, by leaking his location in Pakistan to the US special forces.
"Amal described Khairiah as 'the real killer of bin Laden'. Khairiah accused Amal of sticking to Osama like a prostitute who wanted sex 24 hours a day," the ISI commander, who helped interrogate the pair said.
The two are being held in Pakistan's capital Islamabad, along with a third wife and eight of Bin Laden's 20 kids. Khairiah was said to be jealous his youngest bride got to sleep with him all the time.
Amal, who wed the Al Qaeda chief at 18, was hurt attempting to shield him from US Navy Seals in Abbottabad, according to the same source.
She claimed when Pakistani intelligence grilled her that he knew Khairiah had turned traitor.
Amal says in transcripts of interviews leaked on Wednesdau, "Osama loved me the most. We used to talk about romance, other things apart from Al Qaeda things. In the last days, he felt he was being tracked down by the US forces and their allies. He said his death was to be orchestrated by his wife (Khairiah) or family. He was ready to face it."
An earlier US media report had cited a retired Pakistani brigadier Shaukat Qadir, who carried out his own investigations, in stating that there was "poisonous mistrust" between Osama's three wives, with one of them being accused of betraying him to US intelligence.
Besides the intense jealousies among his wives, Qadir claimed that bin Laden had been sidelined by his outfit. "The Al Qaeda decided to retire him in 2003 as he was mentally senile having picked up some degenerative disease from 2001," he had said.